protein a造句

• We firstly used g protein a subunit as bait
  首先以g蛋白亚基为诱饵构建了诱饵蛋白表达载体。
• Clone and sequence analysis of human helicobacter pylori heat shock protein a gene
  亚单位的基因克隆及序列分析
• Primary application of novel protein a immunoadsorption membrane cartridge to blood purification
  免疫吸附膜柱在血液净化中初步应用
• High efficiency expression and preliminary purification of recombinant heat shock protein a subunit of helicobacter pylori
  亚单位的高效表达及其初步纯化
• Dynamic atomic force microscopic observation of the three - dimensional conformation of protein a - gold single molecule
  胶体金单分子三维构象原子力显微镜研究
• Conjugated protein a protein that on hydrolysis yields not only amino acids but also other organic and inorganic substances
  结合蛋白质：一种蛋白质，它水解后不但能够产生氨基酸而且能够产生其他有机或无机物质。
• Construction , expression and antigenic study of bivalent vaccine candidate with 26 000 omp and heat short protein a of human helicobactor pylori
  人幽门螺杆菌26 000外膜蛋白与热休克蛋白双价疫苗的构建和表达及抗原性研究
• Utilizing the intensity adsorbing effect between the protein a and gold - electrode , and that , the protein a can combined the antibody ' s fc , and it is not influence the active of antibody
  片断结合而不影响抗体的活性，在石英晶振表面固定转铁蛋白抗血清，研制了一种对于转铁蛋白有特殊响应的压电免疫传感器，在s
• On account of vaccination purpose , the neutralizing protection of anti - ns5 antibodies should be tested . ( 3 ) screening antagonistic peptide of ns5 protein a library of phage - displayed disulfide - constrained random peptides was incubated with a plate coated with the recombinant ns5 protein
  ( 3 ) nss蛋白小分子结合肤的筛选:以活性表达的nss蛋白为配基，从噬菌体展示的构象约束性随机肤库中筛选相应的配体分子，共进行了4轮筛选。
• Anti - igg antibody was immobilized onto the surface of gold electrode modified with a thin layer of protein a . biotin labeled anti - igg antibody and hrp - streptavidin were premixed to form network complex to be used as probes for amplifying the sensing response of antibody - antigen interaction
  抗体通过单层蛋白a固定到金电极表面,预先处理好的生物素化抗体和辣根过氧化物酶( hrp )标记的亲和素形成的网状复合物作为免疫反应信号的放大探针。
• Lts membrane properties and effect on cross - iinking biomoiecuiars were discussed . protein a was selected as a sensing membrane owing to the fact that it contains a active spot which can bind fc portion of antibodies . so the orientated immobilization of antibody was achieved
  丁二烯-苯乙烯共聚物作为膜材料，研究了膜的性质和对生物大分子修饰的效果；另外，对具有专一性结合抗体fc功能区的蛋白a作为中间链接层进行了膜性质、定向修饰功能、重复性方面的研究。
• The mechanism of ki 11 ing the schistosomulae in vitro depending on the specific ige antibody were observed . the distribution of the protein encoded by the cloned gene was determined by immuno - electromicroscopy with protein a - colloidal gold . in order to further analyse the epitope features of the protein relevant to the specific ige antibody , the phage display library of random peptides was screened by pooled sera with the specific ige antibody
  借助现行生物信息学分析手段，通过互联网进入genbank数据库，对sj43b与已知序列进行同源性比对，应用dnatoois软件对其编码蛋白序列的氨基酸纽成、亲水性、抗原性和可及性进行分析，并在blastp程序下在genbank蛋白数据库中对sj43b编码3人卞医科大学博士学杠论文蛋白进行同源性检索。
• Protein a and styrene - butadiene - styrene ( sbs ) were selected as sensing membrane to cross iink antibodies ; sbs , poiyvinyi chloride ( pvc ) and poiystyrene ( ps ) are polymers and have many advantages such as easiiy obtained , convenientiy forming membranes and easi1y modified by the introduction of other functional groups . pvc . ps and sbs were used as precoating materials for immobiiizing antibody in our experiment
  选择了两种固定生物大分子的敏感膜：首先，从具有原料易得，成膜方法简单，可以进行表面改性等优点的聚氯乙烯、聚苯乙烯、聚苯乙烯-丁二烯-苯乙烯共聚物三种高分子中选择出成膜迅速、均匀、膜的稳定性好、响应迅速、而且易于控制涂层厚度等优点的聚苯乙烯
• The determination of human thymidine kinase ( htk ) in human serum , which is a key indicator of cancers can give information for the diagnosis and treatment of the malign diseases . the protein a layer was first self - assembled onto the gold electrode surfaces of quartz crystals , the monoclonal antibodies were then orientedly immobilized through the specific binding between the fc terminals of the antibodies and the self - assembled protein a . with this sensor , the affinity constant of antigen - antibody binding was estimated to be 1 . 85 106 l / mol according to the scatchard ’ s plotting method , which proved the high bioactivity of antibody . finally , an amplified piezoelectric immunosensor was designed to determine the htk in
  实验中将蛋白a吸附于镀金压电石英晶体电极表面,用于定向固定htk单克隆抗体,成功研制了检测htk的压电石英晶体传感器,并基于标准scatchard绘图法,计算出免疫反应的亲和常数为1 . 85 106l / mol ,证明该单克隆抗体具有较高的免疫活性;同时基于酶催化沉淀技术,设计了的检测htk的质量放大压电石英晶体传感器,该传感器可在0 . 1 - 10ng范围内对htk进行定量检测,应用此传感器成功地对5种癌症病人血清中htk的浓度进行了测定,实验结果为癌症的临床诊断与治疗提供了参考。
• 二 、 effects of calcium concentration on the membrane - bound gq protein a subunit in the photoreceptor cell of macrobrachium rosenbergi on light adaptation and dark adaptation . the membrane - bound gq a was extracted from the retina of the macrobrachium rosenbergi with protein extract and was electophoresised by sds - page . the percent of the membrane - bound gq a was analyzed by tanon gis gel image disposal system
  二、钙离子浓度对光暗适应罗氏沼虾感光细胞膜结合gq蛋白亚基的影响用蛋白质提取液提取膜结合gq蛋白亚基并sds - page电泳，利用tanongis凝胶图像处理系统分析其含量。
• In our experiment , after light and dark adaptation , the retina of the macrobrachium rosenbergi was respective incubated in high calcium solution , physiological solution and low calcium solution . we studied the effect of calcium concentration on the content and subcellular localization of gq protein a subunit in the photoreceptor cell of macrobrachium rosenbergi on light adaptation and dark adaptation by sds - page technology and imunoelectron microscopy technology . our study results indicated : 一 、 effects of calcium concentration on the soluble gq protein a subunit in the photoreceptor cell of macrobrachium rosenbergi on light adaptation and dark adaptation
  而钙离子对gq蛋白亚基活性有无影响还未见报道。我们以光适应和暗适应条件下的罗氏沼虾复眼视网膜为材料，分别用高钙溶液、生理溶液、低钙溶液孵育后，通过sds ? page电泳技术及免疫胶体金电镜技术，研究钙离子浓度对光暗适应时罗氏沼虾感光细胞gq蛋白亚基含量的影响及亚基亚细胞定位的影响。
• 三 、 effects of calcium concentration on subcellular localization of gq protein a subunit in the photoreceptor cell of macrobrachium rosenbergi on light adaptation and dark adaptation . effects of calcium concentration on subcellular localization of gq protein a subunit in the photoreceptor cell of macrobrachium rosenbergi on light adaptation and dark adaptation were studied using immunogold electronmicroscopy
  三、钙离子浓度对光暗适应罗氏沼虾感光细胞gq蛋白q亚基亚细胞定位的影响采用免疫胶体金电镜技术，研究光暗适应条件下不同钙离子浓度对罗氏沼虾感光细胞中gq蛋白。
• Strain bl21 , and gene expression was induced by iptg . the target proteins were directed into the periplasmic space by the staphylococcal protein a signal sequence preceding the rgd - hirudin gene . using ion exchange chromatography and gel filtration chromatography , the chimera proteins were purified , and both of them showed a single band in tricine - sds - page . the results of activity analysis suggested that these two chimera proteins not only have antithrombin activities , but gain platelet aggregation inhibitory activities as well
  通过离子交换层析和凝胶过滤层分别对两种嵌合体蛋白进行纯化，纯化产物在tricine - sds - page中都显示为单一条带。活性分析结果表明两种嵌合体蛋白在保留水蛭素抗凝血酶活力的同时，还呈现抗血小板聚集活性。
• One was based on the sbs sensitive membrane and " gas phase " immunoassay technology . by immobilized anti - tb or anti - trichinosis on the sbs membrane , it can be used to determine m . tuberculosis and trfohiflosjs . the other one was based on the protein a sensitive membrane and " liquid phase " immunoassay technoiogy
  一种是基于sbs膜固定抗体的压电气相兔疫技术，我们利用该技术首次对结核病和旋毛虫病分别进行了快速诊断：另一种是基于蛋白a定向修饰抗体的压电液相兔疫传感技术，我们利用该技术首次对结核病进行了诊断。
• The detailed investigations are as follows : the first part of the focuses on the development of piezoelectric immunosensor which the immobilization of antibodies with protein a and the study . on kinetics of immuno - reaction using piezoelectric quartz crystal , and the second part is primarily directed to the development of piezoelectric immunosensor which the immobilization antibody with self - assembled monolayers ( sams )
  具体内容如下：本文的第一部分着重于用蛋白a固定抗体的压电免疫传感器的研制以及利用压电石英晶体对免疫反应的动力学研究；论文的第二部分着重于采用自组装技术包埋抗体的压电免疫传感器的研究。